Comparison Between Extracellular Proteins of Mycobacterium Tuberculosis Virulent H37Rv and Attenuated H37Ra
- VernacularTitle:结核分枝杆菌强毒株和弱毒株的胞外蛋白比较分析
- Author:
Xiuyun HE
;
Yuhui ZHUANG
;
Xiaogang ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Extracellular protein;
Differential protein;
Two-dimensional gel electrophoresis separating
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare expression of extracellular proteins of virulent H37Rv and attenuated H37Ra in order to search differential proteins,to provide a train of thought for studing M.TB toxicity further.Methods Extracellular proteins were extracted from H37Rv and H37Ra which were inoculated and cultured on Suton's medium for three weeks.The first dimensional electrophoresis was performed on immobilized pH gradient rod gels(pH 3～10).Then the proteins in the rod gels were separated using SDS-PAGE gels.The silver-stained gels were dried and scanned with image scanner.The 2D image analysis was performed with image Master 2D Elite 3 10.Results The most protein spots deriving from extracellular proteins of H37Rv and H37Ra strains were in acidic range.In the basic range(pI more than 9 0),the number of protein spots belong to extracellular proteins of H37Rv and H37Ra was few.Three protein spots belong to low molecular range in H37Rv strain.However,absent in H37Ra strain.Conclusions Two-dimensional gel electrophoresis is useful to separate protein in Mycobacterium tuberculosis.
