Control of coexpression plasmid of carcinoembryonic antigen and IL-2 gene by internal ribosome entrying site
- VernacularTitle:内部核糖体进入位点控制人癌胚抗原与IL-2的共表达
- Author:
Tianlin HE
;
Chuangang FU
;
Zhongchuan WANG
;
Guisong CAO
;
Qingmin WANG
;
Dan WU
;
Jianxin DAI
;
Shuhan SUN
- Publication Type:Journal Article
- Keywords:
CARCINOEMBRYONIC ANTIGEN/genet;
INTERLEURIN-2/genet;
RIBOSOMES;
GENE EXPRESSION
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo construct the eukaryotic coexpression plasmid CEA/IL-2, and to lay the foundation for further studying CEA nucleiotide vaccine , adjuvant and their effects of special antitumor immunity.MethodsThe eukaryotic expression plasmid (pcDNA3-CEA)containing the gene coding for CEA was obtained by RT-PCR and gene recombination techniques.Using enzymolysis,ligation and other techniques,an eukaryotic coexpression plasmid (pIRES-CEA/IL-2)containing two expression unites of CEA and IL-2 gene connected with internal ribosome site was constructed.ResultsThe coexpression plasmids were transformed into COS7 cells and expression of two proteins were demonstrated by ELISA, and flow cytometer and elecsy.CEA and IL-2 were (23.73?0.26)ng/ml,and(20.17?0.13)ng/ml respectively.ConclusionsThe eukaryotic expression plasmids pIRES-CEA/IL-2 could be successfully constructed and transformed into COS7 cells.Expression of two proteins were demonstrated with no difference on expression.
