Effect of sodium ferulate on expression of bcl-2 and bax in rat lens epithelial cells
- VernacularTitle:阿魏酸钠对大鼠晶状体上皮细胞凋亡相关基因bcl-2和bax的调控
- Author:
Mingxin QI
;
Xiurong HUANG
;
Zhaoyang WANG
;
Yon WANG
- Publication Type:Journal Article
- Keywords:
Sodium ferulate;
Cataract;
Apoptosis;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of sodium ferulate on expression of apoptosis-related genes, bcl-2 and bax , in rat lens epithelial cells (LEC) injured by oxidation.METHODS: Eyes of SD rats were divided randomly into four groups: control group, hydrogen peroxide group (H 2O 2), pirenoxine sodium group (PS) and sodium ferulate group (SF). Eyes were excised and lenses were separated under operating microscope and sterilized condition. Lenses were incubated in CO 2 incubator for 24 h with 300 ?mol?L -1 H 2O 2 and with or rithout 5 mmol?L -1 SF. The expression of Bcl-2 and Bax protein of LEC were measured and compared by tearing the LEC anterior capsule via immunohistochemical analysis.RESULTS: (1) There were Bcl-2 and Bax expression in normal lenses of SD rates, Bcl-2 expression was stronger than Bax. (2) Bcl-2 expression decreased and Bax expression increased markedly ( P
