The effects of propofol on lung nitric oxide synthase activity in rats
- VernacularTitle:异丙酚对大鼠肺一氧化氮合酶活性的影响
- Author:
Ke MA
;
Cai FANG
;
Tao HOU
- Publication Type:Journal Article
- Keywords:
Propofol;
Nitric-oxide synthase;
Lung
- From:
Chinese Journal of Anesthesiology
1997;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol on lung nitric oxide synthase (NOS) in rats and the mechanism of its pulmonary artery and bronchus dilating effects. Methods Forty SD rats weighing (210+40)g were randomly divided into two groups: propofol group received propofol 100mg-kg-1 ip and when its righting reflex was lost propofol was continuously infused at 10mg'kg-1 h-1 through the vein in the tail for 10min, then the animal was killed; control group received normal saline 10 ml'kg-1 ip and the animals were killed 20 min later. After sacrificing the animals the chest was opened and the lungs were removed. The NO level in the broncho-alveolar lavage fluid (BALF) was measured in 10 animals. NOS activity and NO content of the lung tissue homogenate were examined in 10 animals. Cellular distribution and expression of endothelial NOS (eNOS) and neural NOS (nNOS) in the lung were examined by immunohistochemistry in 5 animals. Results The NO level in BALF, NO content and NOS activity in the lung homogenate were significantly higher in propofol group than those in control group (P
