Determination of Contents of Micronomicin by Pre - column Derivatization With RP - HPLC
- VernacularTitle:柱前衍生化反相高效液相色谱法测定硫酸小诺霉素的含量
- Author:
Jian WANG
;
Hui CAO
- Publication Type:Journal Article
- Keywords:
RP- HPLC;
micronomicin;
pre - column derivatization
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method by pre - column derivatization to determine the contents of mi-cronomicin sulfate.METHODS:Kromasil C18 column( 150mm X 4.6mm,5u),mobile phasewater-ice acetic acidmethanol(30 5 : 65) in 0.02mol/L sodium -heptanesulfonate. The detection wavelength was set at 330nm.RESULTS:The linear range was 0.0 976mg/ml-0.9 760mg/ml,(r = 0.9 999),the RSD of the replicate test was 1.0%(n = 6) .CONCLUSION:The method is specific,accurate and sensitive.
