Safety Assessments for Human Skin Fibroblasts Cultured in Vitro
- VernacularTitle:皮肤成纤维细胞体外培养的安全性实验研究
- Author:
Yong CHEN
;
Yuxiang CHEN
;
Zhigao LONG
- Publication Type:Journal Article
- Keywords:
Skin;
Fibroblast;
In vitro culture;
Targeted cell;
Gene therapy
- From:
Journal of Chinese Physician
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Conduct safety assessments of the established human skin fibroblast line. Methods A series of safety assessments were performed including cell morphology,chromosome karyotyping,soft-agar test,nude mice carcinogenic test,endotoxin test,mycoplasma determination,detection of viral agent,bacteria,fungi sterile tests and abnormal toxicity test.Results No abnormal changes were observed.Conclusion The fibroblast obtained from skin is a kind of safety and reliable target cell for gene therapy.
