Inhibitory effect of JIP on AP-1 activity induced by LMP1 in nasopharyngeal carcinoma cells and its mechanism
- VernacularTitle:JIP抑制鼻咽癌细胞中LMP1诱导的AP-1活性及机理的研究
- Author:
Zhi HU
;
Feijun LUO
;
Xiyun DENG
;
Liqun YIN
;
Yan ZHAO
;
Faqing TANG
;
Min TANG
;
Huanhua GU
;
Wei YI
;
Ya CAO
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal neoplasms;
NF-kappa B
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the mechanism of the AP-1 signal transduction pathway inhibited by JIP in nasopharyngeal carcinoma cells. METHODS: AP-1 activity was triggered by Dox-induced LMP1 expression in Tet-on-LMP1-HNE 2 cells (L7). The retention of phospho-JNK in the cytoplasm caused by JIP was examined with immunofluroscence assay. RESULTS: 24 h after transfection of L7 cells with the JIP expression plasmid, the translocation of activated JNK was inhibited, which resulted in the retention of phospho-JNK in the cytoplasm and down-regulation of the AP-1 activity. CONCLUSION: JIP down-regulates the activity of AP-1 through the inhibition of the translocation of JNK.
