Heterogeneity of basal intracellular calcium concentration and its relations to the reactivity in mouse peritoneal macrophages
- VernacularTitle:小鼠腹腔巨噬细胞基态游离钙离子浓度的不均一性及其和细胞反应性的关系
- Author:
Xiaoyan ZHU
;
Jianzhong HAN
;
Shujie LOU
;
Jin YAN
;
Renbao XU
- Publication Type:Journal Article
- Keywords:
Calcium;
Macrophages;
Superoxide;
Mice
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the heterogeneity of basal intracellular free calcium concentration( i) in peritoneal macrophages(PM) and whether it is relative to the reactivity of PM at the single cell level. METHODS: i implicated stimulated were measured by fluorescent microscopic imaging system after loading with fluorescent probe fura-2/AM. Superoxide(O _2)produced by single PM was determined by modified NBT test. RESULTS: The values of basal i determined in 392 PMs of 7 mice showed normal distribution [(54?24) nmol/L, n=392] with wide range(less than 20 nmol/L to more than 100 nmol/L), among which about 50% were in the range of 40-60 nmol/L. When stimulated with PMA or fMLP, i was increased, the peak values were positively correlated with the basal i in one mouse(PMA stimulated cells: r=0.52, P
