Influences of chronic hypoxia on the gene expression of Kv1.3,Kv2.1 and Kv3.1 induced by acute hypoxia in PASMC of rats
- VernacularTitle:慢性缺氧对大鼠肺内动脉平滑肌细胞Kv1.3、Kv2.1、Kv3.1钾通道基因在急性缺氧时表达的影响
- Author:
Zhigang HONG
;
Si JIN
;
Wei KONG
;
Dixun WANG
;
Qiling CHEN
;
Bingyong SUN
- Publication Type:Journal Article
- Keywords:
Pulmonary artery;
Potassium channel;
Anoxia;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646?0.092, 0.782?0.104 to 1.059?0.134, 0.985?0.116,respectively (P
