Activation of renal tubular epithelial cells by monocytes/macrophages and the relative mechanisms
- VernacularTitle:单核巨噬细胞对肾小管上皮细胞的活化作用及其机制初探
- Author:
Li YANG
;
Xiaomei LI
;
Rong WANG
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Macrophages;
Kidney tubules;
Epithelial cells;
Blotting,western;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the direct effects of peripheral blood monocytes/macrophages (MO/MAC) on renal tubular epithelial cells (RTEC),and further probe into the possible mechanisms. METHODS: Conditioned medium(M-CM) of human peripheral blood MO/MAC was collected and added to HK-2,a human renal proximal tubular cell line.After incubation with M-CM for 24 hours,HK-2 cells were detected for DNA synthesis by -TdR incorporation,osteopontin (OPN) and ?-smooth muscle actin (?-SMA) expression by Western blot,and fibronectin(FN) secretion by ELISA.Furthermore,anti-TGF?_1 neutralizing antibody and interlukin-10(IL-10) were used separately to antagonize the effects of M-CM on HK-2 cells. RESULTS: ①DNA synthesis,?-SMA expression and FN secretion were all increased in HK-2 cells when incubated with M-CM.②When adding with anti-TGF?_1 neutralizing antibody (5 mg/L) in the M-CM,the degree of upregulation of ?-SMA and FN in HK-2 cells was much lower than that stimulated by M-CM alone.③M-CM added with IL-10 (20 ?g/L) had a weaker ability to induce the increasing in ?-SMA expression and FN excretion in HK-2 cells, compared with M-CM itself alone.M-CM from MO/MAC preincubated with IL-10 caused a lower upregulation of ?-SMA expression in HK-2 cells than M-CM from non-preincubated MO/MAC. CONCLUSION: MO/MAC can directly induce proliferation,transdifferentiation and extracellular matrix secretion in RTEC.TGF?_1 and proinflammatory cytokines secreted by MO/MAC might be involved in the aboveeffects.
