The protective effect of anti-macrophage migration inhibitory factor monoclonal antibody on oleic-acid-induced acute lung injury
- VernacularTitle:抗巨噬细胞移动抑制因子抗体在油酸致急性肺损伤发病中的干预作用
- Author:
Yubiao GUO
;
Canmao XIE
;
Zhiping LI
;
Huiling YANG
- Publication Type:Journal Article
- Keywords:
Lung/injuries;
Macrophage migration inhibitory factor;
Intercellular adhesion molecule-1;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the protective effect of anti-macrophage migration factor monoclonal antibody (anti-MIF MAb) on oleic-acid-induced acute lung injury (ALI) rats and its influence on the expression level of MIF and intercellular adhesion molecule-1(ICAM-1). METHODS: The rats were subjected to injection of oleic acid (oleic acid group) or saline solution (control group). One hours before administration of oleic acid, the rats were intraperitoneally injected with anti-MIF antibody (5 mg/kg) as the treatment group. After injecting oleic acid or saline for 4 hours, the PaO_2, lung permeability index (LPI), the number of macrophage and the level of soluble ICAM-1 (sICAM-1) in the bronchial alveolar lavage fluid (BALF) were measured. The expression level of MIF mRNA and ICAM-1 mRNA in the lung were detected by in situ hybridization, and the degree of macrophage infiltration and the expression of MIF were evaluated by double staining immunocytochemistry. RESULTS: The PaO_2 of the oleic acid group was far lower than those of the control and treatment group (P
