The effect of hypertonic-hyperoncotic solution on CD11b expression on the lipopolysaccharide-stimulated human neutrophils in vitro
- VernacularTitle:高晶体-高胶体渗透压混合液对脂多糖刺激的人中性粒细胞表面粘附分子CD11b表达的影响
- Author:
Xueping ZHANG
;
Shiying YUAN
;
Yonggong REN
- Publication Type:Journal Article
- Keywords:
Hypertonic solutions;
Neulrophils;
Cell adhesion molecules
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hypertonic-hyperoncotic solution (HHS) on CD11b expression on the surface of human neutrophils which are stimulated by lipopolysaccharide (LPS) in vitro. Methods Neutrophils were isolated from fresh peripheral venous blood of healthy volunteers aged 20-4oyr find incubated with LPS 100 ng/ml al 37℃ in 5% CO2 incubator for 15, 30, 60min, 4, 12 or 24h. HHS was prepared with 10%NaCl and 10% hydroxyethyl starch. Neutrophils were incubated with 0.25% or 0.5%HHS for 10min, 1h or 4h. Some of the HHS treated neutrophils were further subjected to LPS stimulation for 30 mm. CDllb on the surface of neutrophils were measured by using immunofluorescence and flow cytometry. Results CD11b expression on neutrophils increased significantly after being exposed to LPS for 15min (P
