Effects of propofol in TNF-induced apoptosis in endothelial cells derived from human umbilical vein
- VernacularTitle:异丙酚对肿瘤坏死因子诱导的内皮细胞凋亡的影响
- Author:
Tao LUO
;
Zhongyuan XIA
;
Xuejun CHEN
- Publication Type:Journal Article
- Keywords:
Propofol;
Tumor necrosis factor;
Apoptosis;
Endothelium, vascular
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of propofol in clinical relevant concentration on TNF-induced apoptosis in endothelial cells derived from human umbilical vein (HUVECs) .Methods The third and fourth passages of the cultured endothelial cells were divided into 5 groups. 0, 12.5, 25, 50 or 100 ?mol/L propofol was added to the cultured cells respectively and the cells were incubated for 30 min. Then TNF-? was added to the cultured cells (the final concentration of TNF-? was 2000 U.ml-1 ) which was incubated for 24h. Apoptosis was detected by using "terminal deoxynucleotidyl transferase (tdt )-mediated deoxyuridine triphosphate (dUTP )-biotin nick end-labelling" (TUNED . Morphologic changes were examined by means of electron microscope.Results Apoptosis expression was very high in control group (no propofol was added) . The process can be inhibited by pre-treatment with propofol (P
