Rapid Identification of Candida albicans and Candida dubliniensis Isolates With Two Specific Probes Si-multaneously
- VernacularTitle:微孔板双探针杂交法快速鉴别都柏林念珠菌和白念珠菌
- Author:
Liping ZHU
;
Qiangqiang ZHANG
;
Yaozhong SHI
;
Li LI
;
Jiajun WANG
;
Xinhua WENG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Candida dubliniensis;
Nucleic acid hybridization;
DNA pr obe
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a microtitration plate en zyme immunoassay to differentiate Candida albicans from Candida dubliniensis isolates using two specific probes s imultaneously.Methods The fun-gus-specific universal primers derived from the internal transcribed s pacer region of fungal rDNA were labeled with biotin,while the C.albicans or C.dubliniensis specific capture probes were coated on the microplates.Genomic DNA purified from the two species was amplified by PCR.The biotinylated p roducts were captured by the probes coated on the microplates.The A 405 value was finally determined by the c olorimetric assay.Results The two species of Candida could be detected specifically.Out of 108clinical isolates originally identified as C.albicans on the basis of germtube formation,two isolates were positive for C.dubliniensis and negative for C.albicans.The other106isolates were positive for C.albicans and negative for C.dubliniensis.Conclusions Two-specific-probe hybridization method is rapid and re liable for differentiating C.albicans from C.dubliniensis.
