Effects of growth hormone on apoptosis of intestinal mucosal cell in rats with acute necrotizing pancreatitis
- VernacularTitle:生长激素对急性坏死性胰腺炎大鼠肠粘膜上皮细胞凋亡的影响
- Author:
Xingpeng WANG
;
Bingxian WANG
;
Kai WU
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
Apoptosis;
Somatropin
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was to evaluate the effects of growth hormone (GH) on cell apoptosis of intestinal epithelium in acute necrotizing pancreatitis (ANP) rats.Methods Seventy-two SD rats were divided into three groups randomly: sham operation (SO) group, ANP group, and GH treatment group. GH-treatment group received subcutaneously 0 75?U/kg of GH. At 3?h, 6?h, 12?h, and 24 ?h after induction of ANP, the small intestinal specimens were harvested and apoptosis of intestinal epithelium was studied by DNA gel electrophoresis, FCM and TUNEL method. FasL and Bax protein expressions were detected by immunohistochemistry.ResultsThe ladder pattern on DNA gel electrophoresis presented in all time points of ANP group, but only seen in GH-treated group at 3h. Apoptotic percentage significantly increased in ANP group [(50?11)%,(80?9)%,(48?17)%,(50?10)%] as compared with SO group [(54?4)%, (28?6)%, (39?5)%,(29?11)%], all P
