Detection of micrometastasis in peripheral blood of patients with colorectal carcinoma before and during operative procedure
- VernacularTitle:大肠癌患者术前和术中外周血微转移的检测及其临床意义
- Author:
Guole LIN
;
Huizhong QIU
;
Tong XU
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Keratin;
Micrometastasis;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the relationship between surgical manipulation and hematogenous spreading micrometastasis in patients with colorectal carcinoma.MethodsNested RT-PCR was used to detect the expression of cytokeratin 20 (CK20) mRNA in the peripheral blood of 37 colorectal cancer patients without distal metastasis (experimental group) undergoing radical resection which were subdivided into group A (tumor drainage veins were first ligated) and group B (without precedent ligation of the veins).Results CK20 mRNA was positive by nested RT-PCR in the peripheral blood in 9 out of 10 colorectal cancer patients with known distant metastasis,while it was negative in all 10 volunteers and all 10 patients with benign colorectal lesions. CK20 mRNA was detected in 14 of 37 (37 8%) cases in the peripheral blood sampled preoperatively, while the positive ratio rose to 59 5% (22/37) during surgical procedures (? 2=4 900, P0 05). ConclusionCK20 mRNA by nested RT-PCR was highly sensitive and specific for the determination of circulating micrometastasis in colorectal cancer patients. Surgical manipulation significantly increased the incidence of hematogenous spreading micrometastasis, which can′t be prevented by precedent ligation of the refluent veins of the tumor during operation.
