Effect of paclitaxel on telomerase activity, apoptosis and p53/bcl-2 gene protein expression of breast cancer MCF-7 cell line
- VernacularTitle:紫杉醇对乳腺癌MCF-7细胞端粒酶、凋亡及p53/bcl-2表达影响的研究
- Author:
Rongqing PANG
;
Buzhen ZHANG
;
Xinghua PAN
- Publication Type:Journal Article
- Keywords:
Genes, p53;
Genes, bcl-2;
Paclitaxel;
Telomerase;
Apoptosis
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect on telomerase activity, apoptosis and p53/bcl-2 gene expression of MCF-7 cells line induced by paclitaxel.Methods By techniques of cell culture in vitro, telomeric repeat amplification protocol with ELISA(TRAP-ELISA) and flow-cytometry (FCM), MCF-7 cell line was treated by paclitaxel in various concentration for 72h.Results Paclitaxel down-regulated telomerase activity of MCF-7 cell, induced apoptosis of the cell in a concentration-dependent manner, significantly decreased expression of bcl-2 gene protein and increased expression of p53 gene protein. There was a positive correlation between telomerase activity and apoptosis and the expression of p53/bcl-2 gene protein.ConclusionPaclitaxel could down-regulate telomerase activity,induce apoptosis, decrease expression of bcl-2 gene protein and increase expression of p53 gene protein, which may be one of important mechanisms of Paclitaxel′s anticancer action.
