Simultaneous Determination of Chloromycetin and Dexamethasone Acetate in Jiefu Cream by HPLC
- VernacularTitle:高效液相色谱法同时测定洁肤霜中氯霉素与醋酸地塞米松的含量
- Author:
Yanan WANG
;
Xiongping JIANG
;
Runjie ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
chloromycetin;
dexamethasone acetate
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the contents of chloromycetin and dexamethasone acetate in Jiefu cream.METHODS:A reversed-phase HPLC was established for the determination with C18 column as stationary phase.The mobile phase consisted of acetonitrile and 0.05%mol/L phosphate buffer solution(40∶60),the detection wavelength was 240nm.RESULTS:Chlorom-ycetin had a good linearity (r=0.9 998) in the range of 0.1～0.3mg/ml,the mean recovery was 100.6% with a RSD of 1.3%.Dexamethasone acetate had a good linearity(r=0.9 998) in the range of 0.02～0.08mg/ml,the mean recovery was 101.1% with a RSD of 1.7%.CONCLUSION:The method is simple,sensitive and accurate.
