Detection of Mutations in the Wilms' Tumor Gene (WT1) in Leukemias
- VernacularTitle:白血病细胞WT瘤基因突变的检测
- Author:
Qing LU
;
Gexiu LIU
;
Jianying HAN
- Publication Type:Journal Article
- Keywords:
Leukemia;
Wilms tumor gene;
Mutation;
PCR-Single-strand conformational polymorphism
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze mutations in the Wilms tumor gene (WT1) in Ieukemogenesis.Methods WT1 gene in peripheral blood was determined by PRC-SSCP technique,in 32 cases of acute leukemia,included 9 cases of acute granulocytic leukemia,8 cases of chronic granulocytic leukemia(mean age-33 years) and 16 specimens of normal subjects were also detected.Results Mutations in the WT1 gene in 3 of 32 leukemias were found .WT1 mutations were found in 11% of cases of acute lymphoblastic leukemia and in 13% of cases of acute myeloid leukemia,in which they were associtated with a poor response to chemotherapy.Conclusions The mutations in Wilms tumor gene WT1 are associated with leukemogenesis and its therapy,which WT1 transcripts may prove a significant tumor marker, as a MRD monitor in evaluating remission status and early relapse,and may be useful in prognosis of acute leukemia.
