Effects of acute and chronic hypoxia on rat brain mitochondrial translation activity
- VernacularTitle:急、慢性低氧对大鼠脑线粒体蛋白翻译合成的影响
- Author:
Mingchun CAI
;
Junze LIU
;
Liping WU
;
Bingyon SUN
- Publication Type:Journal Article
- Keywords:
WTBZ]Mitochondria;
Translating;
Anoxia;
Rats
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of simulated high altitude hypoxia on rat brain mitochondrial translation activity. METHODS: Animals were continuously exposed to simulated 4 000 m high altitude in hypobaric chamber for three days and forty days. Mitochondria of rat brain were isolated by homogenizing brain tissue and following centrifuging program. Protein translation activity in isolated mitochondria in vitro was measured with -Lencine incorporation method. Products labeled with -methionine in isolated mitochondrial protein synthesis system in vitro were separated on SDS-PAGE and identified by autoradiography. RESULTS: Mitochondrial translation activity in vitro in acute hypoxia exposure were significantly lower than control( P
