ATPase activities and Ca~(2+)/calmodulin alterations in hippocampi of rats with PTSD-like behaviors
- VernacularTitle:PTSD样情感行为异常大鼠海马ATP酶活性与Ca~(2+)/CaM改变
- Author:
Qingsong WANG
;
Zhengguo WANG
;
Peifang ZHU
- Publication Type:Journal Article
- Keywords:
Stress disorders, post-traumatic;
Adenosinetriphosphatase;
Calcium;
Calmodulin;
Hippocampus
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the pathophysiological bases in the pathogenesis of the lasting emotional behavioral disorders following posttraumatic stress disorder (PTSD). METHODS: 240 male Wistar rats were divided randomly into 3 groups. Group SE ( n =96) for rats with PTSD-like behavior by constant pulsating current of 100 ?A with intratrain frequencies of 16 Hz, pulsating duration of 1 ms, train duration of 10 s and interstimulus interval of 7 min for 5 days with 8 times per day. Group CE ( n =96) for control with electrode implanted in hippocampus without stimulation, and Group NC ( n =48) for normal control. The activities of Na +-K +-ATPase and Ca 2+ -ATPase, levels of intracellular calcium and free calmodulin (CaM), and the total CaM expression were detected in hippocampi of experimental rats. RESULTS: The activities of Na +-K +-ATPase and Ca 2+ -ATPase in mitochondria of hippocampal cells in Group SE rats were significantly decreased at 48 h and 72 h after the last stimulation, respectively. The intracellular free calcium levels were increased, and the mean channel fluorescence of intracellular free CaM decreased remarkably at 72 h poststimulation, while the expression of total CaM was significantly elevated at 48 h after the last stimulation in hippocampi of Group SE rats. CONCLUSION: The lasting increased levels of intracellular free calcium and expression of Ca 2+ -CaM in hippocampus, as well as the dysfunction of Na +-K + pump and Ca 2+ -ATPase in mitochondria may play important roles in the long-term neuropsychological sequelae in PTSD.
