Effect of diltiazem on pulmonary arterial pressure and ceNOS mRNA expression in pulmonary arteries in chronic hypoxic hypercapnic rats
- VernacularTitle:硫氮卓酮对慢性低O_2高CO_2大鼠肺动脉压力和结构型一氧化氮合酶及其基因表达的影响
- Author:
Liangxing WANG
;
Shaoxian CHEN
;
Zhengjie XU
;
Linsheng YU
;
Qunji WANG
;
Yupeng XIE
;
Yanfan CHEN
;
Wei WANG
;
Hongqin ZHANG
- Publication Type:Journal Article
- Keywords:
Diltiazem;
Anoxia;
Hypercapnia;
Pulmonary artery;
Nitric oxide synthase;
Gene
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of diltiazem on mean pulmonary arterial pressure(mPAP) and nitric oxide synthase(NOS) in arterioles in chronic hypoxic hypercapnic rats. METHODS: Twenty-four rats were randomly divided into three groups: control group(A),hypoxic hypercapnic group(B), hypoxic hypercapnia+ diltiazem group (C), constitutive endothelial NOS(ceNOS) were observed in arterioles of rats using the technique of immunohistochemistry,ceNOS mRNA were observed by the technique of in situ hybridization . RESULTS: (1)mPAP was significantly higher in rats of B group than that of A and C group( P 0 05),but mCAP was lower in rats of C group than that in B group.(2)Light microscopy showed WA/TA (vessel wall area/total area) was significantly lower in rats of C group than that of B group ( P
