Study on the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes of mouse
- VernacularTitle:小鼠胚胎干细胞分化心肌细胞钠通道、钙通道电生理特性研究
- Author:
Xiang WANG
;
Qiutang ZENG
;
Wuqiang ZHU
;
Linsheng CAO
- Publication Type:Journal Article
- Keywords:
Embryo;
Stem cells;
Myocardium;
Electrophysiology;
Mice
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes (SCDC) of mouse. METHODS: Embryonic stem cells of D 3 line (ES-D 3) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D 3 cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D 3 cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC.
