The impact of scavenger receptor class A typeⅠ/Ⅱ on lipid metabolism in mice
- VernacularTitle:清道夫受体AⅠ/Ⅱ对小鼠脂质代谢的影响
- Author:
Wenjian WANG
;
Xueqing YU
;
Xiaoyan LI
;
Xunhua ZHENG
;
Min XIA
;
Wenhu LING
- Publication Type:Journal Article
- Keywords:
Lipid metabolism;
Genes;
Mice
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the impact of scavenger receptor class A type Ⅰ/Ⅱ (SR-A Ⅰ/Ⅱ) on the lipid metabolism in SR-A Ⅰ/Ⅱ gene deficient mice. METHODS: A probe of 660 bp fragment of SR-A Ⅰ/Ⅱ cDNA digested with PstⅠ and XhoⅠ from plasmid 122 was used to identify whether SR-A Ⅰ/Ⅱ had been knocked out in the tail DNA of the mutant (SR-/-) and control (SR+/+) mice by the method of Southern-blot analysis. The serum levels of triglycerides(TG), cholesterol(CH), low density lipoprotein(LDL), high density lipoprotein (HDL), apolipoprotein (Apo) A and ApoB of the mice fed with normal food and higher lipid food respectively were tested by biochemical method. RESULTS: The serum levels of LDL and body weights of group with SR-A Ⅰ/Ⅱ gene knocked out were higher than that of control group ( P
