Study on the binding capacity of IgA_1 from patients with IgA nephropathy to human mesangial cells
- VernacularTitle:IgA肾病患者血清IgA_1与正常人肾小球系膜细胞结合动力学研究
- Author:
Yue WANG
;
Minghui ZHAO
;
Youkang ZHANG
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Glomerulonephritis, IGA;
Carrier proteins
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine and compare the ability of serum IgA 1, from both the patients with IgA nephropathy (IgAN) and the healthy control, to bind to human mesangial cells (HMC). METHODS: Serum IgA was isolated with jacalin column, heated to aggregated form (aIgA 1) and labeled with [ 125 I]. Binding capacity of aIgA 1 to primary HMC was evaluated by radioligant binding assay, specificity of binding was determined by competitive inhibition, and relative affinities was compared by cross competitive inhibition. RESULTS: Both aIgA 1 from normal control and patients with IgAN bound to MC in a dose-dependent, saturatable manner, but the binding of aIgA 1 from patients was saturated at approximately 200 pmol while that from healthy was at 400 pmol. The Scatchard analysis revealed a Kd of (8 9?2 1)?10 -8 mol/L for patient's aIgA 1 versus (4 3?1 2)?10 -7 mol/L for normal aIgA 1 ( P
