Effects of Ca~(2+)on the releases of ET-1, NO and PGI_2 from bovine coronary artery endothelial cells during hypoxia
- VernacularTitle:低氧时钙离子对冠状动脉内皮细胞释放ET-1、NO、PGI_2的影响
- Author:
Weigong LIAO
;
Zengzhu XIE
;
Shumin XU
;
Yufeng ZHOU
;
Qiang WAN
- Publication Type:Journal Article
- Keywords:
Anoxia;
Verapamil;
Endothelins;
Nitric oxide;
Epoprostenol
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the mechanism of ET-1, NO and PGI 2 release from coronary artery endothelial cells (CAEC) induced by acute hypoxia. METHODS: Bovine coronary artery endothelial cells were cultured and [ 45 Ca 2+ ] was used to investigate the difference of calcium uptake between normoxia group and hypoxia group (3% O 2). The contents of ET-1, NO and PGI 2 in media of normoxia group, hypoxia group and hypoxia + verapamil group were measured 24 h after hypoxia. RESULTS: [ 45 Ca 2+ ] uptake by CAEC in hypoxia group was 1.9 times more than normoxia group ( P
