Effect of homocysteine on secretion and expression of interleukin-6 in cultured rat vascular smooth muscle cells
- VernacularTitle:同型半胱氨酸对大鼠血管平滑肌细胞分泌和表达IL-6的影响
- Author:
Jianhua ZHU
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Interleukin-6;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of homocysteine (Hcy) on secretion and expression of interleukin-6 (IL-6), which is a multifunctional proinflammatory cytokine, in cultured rat vascular smooth muscle cells (VSMCs). METHODS: Rat VSMCs were stimulated with Hcy. Cell ELISA was performed to measure the expression of IL-6 protein and semiquantitative RT-PCR was used to dectect the IL-6 mRNA expression. RESULTS: Compared with control, treatment of 0.25 mmol Hcy for 6 h could increase IL-6 production. In addition, Hcy concentration-dependently increased the expression of IL-6 protein in these cells. 0 1 mmol/L, 0 25 mmol/L Hcy increased IL-6 production 1 4-fold and 3 4-fold, respectively Furthermore, RT-PCR analysis demonstrated that homocysteine also enhanced IL-6 mRNA expression in a concentration- and time-dependent manner.CONCLUSION: Homocysteine can induce IL-6 expression in VSMCs and elicit vascular inflammatory response, which may thereby influence the pathogenesis of atherosclerosis.
