Effects of angiotensin receptor antagonist Losartan on the expression of transforming growth factor (TGF) ?_1mRNA in cirrhotic rats
- VernacularTitle:血管紧张素Ⅱ受体拮抗剂对肝硬化大鼠TGF?_1基因表达影响的实验研究
- Author:
Lixin LI
;
Yu WANG
;
Zhongtao ZHANG
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis;
Angiotensin Ⅱ;
Transforming growth factor beta
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of angiotensin Ⅱ receptor antagonist Losartan on the expression of TGF ? 1mRNA in rats with cirrhosis and the mechanism.Method Forty one male SD rats were divided into normal control ( n =10), cirrhotic control ( n =11), and Losartan treated cirrhotics ( n =20) with Losartan (10?mg?kg -1 ?d -1 ) treatment started on the first day along with CCl 4 administration and on the 5th week, respectively.Expression of TGF ? 1 mRNA was examined with reverse transcriptase polymerase chain reaction (RT PCR).The expression of TGF ? 1 protein was assayed with immunochemistry.Result Expression of TGF ? 1 mRNA and TGF ? 1 protein were up regulated in rats with cirrhosis (from 0 93?0 05 to 1 94?0 13, compared with the normal rats P
