Expression and activation of transcription factor E2F1 in cultured human retinal pigment epithelial cells induced by serum
- VernacularTitle:血清诱导人视网膜色素上皮细胞中转录因子E2F1的表达及活化
- Author:
Xiaoqing LI
;
Shuiqing ZENG
;
Lili XU
- Publication Type:Journal Article
- Keywords:
Transcription factor;
Pigment epithelium of eye;
Blotting,western
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and activation of transcription factor E2F1 in cultured human retinal pigment epithelium (RPE) cells. Methods Cultured human RPE cells were divided into two groups after synchronization: one was cultured in Dulbecco′s modified Eagle′s medium (DMEM) without serum; the other was cultured in DMEM supplemented with 20% serum of newborn calf. The expressions of E2F1 protein in two groups were detected by Western blot analysis. The E2F1 DNA binding activities were measured by gel mobility shift assay(EMSA). Results E2F1 protein of 60 000 molecular weight was detected in the nuclear extract of human RPE cells, and serum stimulation could increase its expression( P
