Effect of activation of stimulator cells on expression of CD69 by responder T cells in mixed lymphocyte reaction
- VernacularTitle:MLR中刺激细胞活化状态对应答T细胞CD69表达的影响
- Author:
Jingxian ZHAO
;
Yaoying ZENG
;
Xianhui HE
;
Jingfang DI
;
Shan ZENG
;
Nan WANG
- Publication Type:Journal Article
- Keywords:
Leukocyte culture test, mixed;
Activation analysis;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the influence of status of stimulator cells on activation of responder T cells in mixed lymphocyte reaction (MLR), so as to provide some basis for clinical transplantation. METHODS: Stimulator cells were pretreated differently before mixed lymphocyte culture (MLC) to change their functional status, fluorescence conjugated antibodies and flow cytometry were used to detect expression of CD69 by responder T cells at several different time points. RESULTS: The expression percentages of CD69 by responder T cells in MLCa group (stimulator cells were pre-activated) were significantly higher than those in MLC group (stimulator cells were not pre-activated) at 24, 48 and 72 hours of culture, respectively (5 21%?0 24% vs 1 98%?0 33%, 29 81%?0 85% vs 20 65%?1 00% and 39 61%?1 62% vs 13 49%?0 60%, P
