Changes of IL-10 mRNA expression and activator protein-1 activity in lung of rats with SIRS-acute lung injury induced by lipopolysaccharides
- VernacularTitle:全身炎症反应综合征-肺损伤大鼠肺组织IL-10mRNA表达及AP-1活性变化的研究
- Author:
Qi LI
;
Guisheng QIAN
;
Qing ZHANG
;
Zhengcai TANG
;
Zhengji GAO
- Publication Type:Journal Article
- Keywords:
Respiratory distress syndrome;
Lipopolysaccharides;
Interleukin-10;
RNA, messenger
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the model of systemic inflammatory response syndrome (SIRS)/acute lung injury (ALI) by LPS administration in rats and to measure the content of interleukin-10 massage RNA(IL-10 mRNA) and activator protein-1 (AP-1) activity in order to investigate the anti-inflammatory mechanism.METHODS: Wistar rats were injured with increased dose of LPS to set up the SIRS/ALI model. Reverse transcriptase-polymerase chain reaction(RT-PCR) and electrophoretic mobility shift assays (EMSAs) were used to measure IL-10 mRNA content and the AP-1 binding activity in rat lung, respectively.RESULTS: ①LPS could be applied to simulate SIRS-ALI in rats.②Acute respiratory distress syndrome (ARDS) could be induced under condition of LPS≥6 mg/kg, which was similar to that of the excessive expression of SIRS. ③ LPS may cause the increase content of IL-10 mRNA and AP-1 activity in lung in rat. ④Content of IL-10 mRNA and AP-1 activity were increased significantly under LPS≥6 mg/kg. CONCLUSIONS: ①LPS≥6 mg/kg might cause the SIRS/acute lung injury in rats.②The rats with excessive SIRS/acute lung injury had an obvious increase of transcription of IL-10 gene and upregulated AP-1. ③ Intensified anti-inflammatory mechanism plays a pathological role in SIRS/acute lung injury.
