Effect of IL-10 on IL-1?-induced cyclooxygenase-2 expression and prostaglandin E_2 release in human mesangial cells
- VernacularTitle:白细胞介素-10抑制人肾系膜细胞环氧化酶-2表达及其催化的前列腺素E_2释放的实验研究
- Author:
Yuanjun WU
;
Ronghua CHEN
;
Jiqing CHEN
;
Aihua ZHANG
;
Xiaoqi PAN
- Publication Type:Journal Article
- Keywords:
Interleukin-10;
Interleukin-1;
Prostaglandins E;
Prostaglandin-endoperoxide synthase
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of IL-10 on IL-1?-induced prostaglandin E 2(PGE 2) release and cyclooxygenase-2(COX-2) expression in human mesangial cells and to examine whether IL-10 has effect on the biological function of IL-1?.METHODS: The PGE 2 concentration in supernatants of HMC was measured by radioimmunoassay. The COX-2 mRNA and protein were measured by RT-PCR and Western blot, respectively. RESULTS: PGE 2 and COX-2 were significantly increased after treatment with IL-1?( P0.05 , respectively), while it inhibited IL-1?-elicited PGE 2 production, as well as COX-2 mRNA and protein expression in a concentration-dependent fashion. CONCLUSIONS: These results indicated that IL-10 depressed the IL-1?-induced release of PGE 2 and expression of COX-2. These data suggested that IL-10 could exert anti-inflammatory actions at several levels, not only by inhibiting the production of pro-inflammatory cytokines but also by suppressing their biological function.
