Whole cell screening of phage-display peptide library for mimicry peptides of glioma SWO-38
- VernacularTitle:利用噬菌体随机肽库对神经胶质瘤母系SWO-38进行全细胞筛选
- Author:
Shuangrong GAO
;
Xueyun ZHONG
;
Yanling YAN
;
Zhenyu ZHONG
;
Yanfang QIN
;
Yanhua SUN
;
Bing WANG
;
Lina YU
;
Ying ZHONG
- Publication Type:Journal Article
- Keywords:
Phage;
Glioma;
Clone cells.
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS:The Ph.D.-7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.Moreover,binding efficiency analysis was carried out to test the binding specificity of the clones obtained. RESULTS: After three rounds of biopanning,a high concentration of phage clones was obtained and two of them were found to be highly specific to glioma SWO-38. CONCLUSION: Highly specific clones against neurtral glioma cells can be obtained from a phage display library by simple procedures.
