Effects of TNF-?,sTNFRⅠon IL-8Production and Proliferation of Psoriatic Keratinocytes
- VernacularTitle:肿瘤坏死因子?对角质形成细胞产生白介素8和增殖活性的影响
- Author:
Xianjin CHEN
;
Kaiming ZHANG
;
Guohua YIN
;
Huixian LI
- Publication Type:Journal Article
- Keywords:
Psoriasis;
Tumor necrosis factor;
I nterleukin -8;
Keratinocyte
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of TNF -?and sTNFRⅠon IL -8production and proliferatio n of psoriatic keratinocytes.Methods In vitro cultured keratinocytes were treated with TNF -?,sTNFRⅠand an-ti -TNFRⅠ.IL -8levels in supernatants were me asured by enzyme-linked immunosobent assay.Expression of IL -8mRNA in keratinocytes was observed by semiquantitive RT -PCR and Southern blotting.Proliferation index wa s measured by MTT colorimetry.Results IL -8levels,mRNA expression and pro liferation index were significantl y higher in psoriatic patients than th ose in control group(P
