The Effects of Ethanol Extracts of Chinese Medicines on the Growth and Proliferation of Dermal Fibroblasts from Human Hypertrophic Scar in Vitro
- VernacularTitle:五倍子等对体外培养的人皮肤瘢痕组织成纤维细胞生长的影响
- Author:
Danbei SHEN
;
Qiang JU
;
Jihai SHI
- Publication Type:Journal Article
- Keywords:
Cicatrix,hypertrophic;
Fibroblasts;
Carthamus tinctorius;
Galla chi nensis;
Scolopendra subspinipes
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Chinese traditional medicines on human hypertrophic scar fibroblasts in vitro,and determine their effective dosage.Methods Traditional Chinese medicines were cultured with human hypertrophic scar fibrob lasts(Fb)and normal dermal Fb,MTT colorimetr ic assay and cell growth curve were used to compare the influence of ethanol extracts fromChinese tradi tional medicines on the Fb growth activity and growth cycle.Results(1)Chinese gall markedly inhibited the proliferation of Fb derived from hyp ertrophic scar and normal skin,and was more effective on the former in a dose-dependent m anner(0～400?g /mL)during 72h of treatment.(2)The half inhibition concentration o f Chinese gall ethanol extract was 100?g /mL.The morphology of Fb did not change when its concentration was below 400?g /mL,as compared to colchicine(
