Effects of tumor necrosis factor-? on skeletal muscle ischemia reperfusion injury
- VernacularTitle:肿瘤坏死因子?介导骨骼肌缺血-再灌注损伤的实验研究
- Author:
Qingfeng ZHENG
;
Pingfan GUO
;
Fuzhen CHEN
- Publication Type:Journal Article
- Keywords:
Muscle, skeletal;
Reperfusion injury;
Tumor necrosis factor
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the effect of tumor necrosis factor ? on skeletal muscle ischemia reperfusion injury (SMIR). Methods Twenty four healthy male Sprague Dawley rats were divided into three groups. Group A underwent anesthesia and external jugular vein cannulation only. Group B underwent 4 hours of left hind limb ischemia followed by 4 hour reperfusion. Group C ischemia and reperfusion were treated with anti TNF ? monoclonal antibody (2?mg/kg). ResultsSMIR significantly increased the transcription of TNF ? mRNA in monocyte. The increased TNF ? raised significantly the level of MDA(9 9?1 8 vs. 5 5?0 4)?CK(122?24 vs. 49?11)?NO(270?98 vs. 128?46) in plasma and MPO(skeletal muscle 4 27?0 53 vs. 1 28?0 19, lung 2 61? 0 12 vs. 0 57?0 02) in tissues respectively( P
