Determination of Prednisolone and Prednisolone-hemisuccinate in Biological Media by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定生物介质中泼尼松龙及泼尼松龙琥珀酸半酯
- Author:
Jin ZHOU
;
Pin LI
;
Jianfeng CHENG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
prednisolone;
prednisolone-hemisuccinate
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an analytical method for the determination of prednisolone and prednisolone-hemisuccinate in biological media METHODS:Using RP-HPLC,biological media were extracted with a mixture of n-pentane and methyl tert-butyl ether(2∶3) The mobile phase consisted of acetonitrile and trisodium citrate buffer (33∶67) The sample was eluted on C18 column and detected at 254nm RESULTS:The extraction recoveries of two drugs were over 80% and the RSDs of within-day and day-to-day were below 3% CONCLUSION:The method is simple,rapid,sensitive,accurate and good enough to be applied to the pharmacokinetic study of new preparation of prednisolone and can also be applied to other similar glucocorticoids
