Determination of Chloramphenicol and Dexamethasone Sodium Phosphate in Ear Drops by HPLC
- VernacularTitle:高效液相色谱法同时测定氯地滴耳液中主药的含量
- Author:
Diankun HUANG
;
Peng LEI
;
Zhiyong DAI
- Publication Type:Journal Article
- Keywords:
HPLC;
chloramphenicol;
dexamethasone sodium phosphate
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of chloramphenicol and dexamethasone sodium phosphate in ear drops by HPLC METHODS:Chloramphenicol and dexamethasone sodium phosphate were separated with a mobile phase of methanol-phosphate buffer solution(0 34% KH2PO4 solution)(60∶40,v/v,1 0ml/min)on Kromasil C8 column and detected at 240nm RESULTS:Good linearity appeared in the concentration of chloramphenicol 100～500?g/ml,r=0 9 999,the recovery was 98 5%～100 8%,RSD≤1 5%;and good linearity showed in the concentration of dexamethasone sodium phosphate 16～80?g/ml,r=0 9 990,the recovery was 98 8%～100 6%,RSD≤2 0% CONCLUSION:This method can simultaneously detect both ingredients in the ear drops
