Establishment of Model of Diffuse Mesangioproliferation Complicated With Focal Segmentally Glomerulosclerosis Model of Rat
- VernacularTitle:弥漫性系膜增生伴局灶节段性肾小球硬化大鼠模型的建立
- Author:
Hao ZHAN
;
Longzhen JI
;
Kun WU
- Publication Type:Journal Article
- Keywords:
Mesangioproliferation;
Glomerulosclerosis;
Glomerulonephritis;
Animal model,rat
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a animal model for diffusion mesangioproliferation complicating focal segmentally glomerulosclerosis.Methods The models were established in the seventieth day by repetitive injection of 4mg/kg and 3mg/kg adriamycin to the SD rats in the 7th and 30th day after removal of their right kidneys.Results Serious edema, profuse albuminuria, hypoalbuminemia, hyperlipemia and renal failure were presented. Simultaneously, diffuse medium- or serious-grade proliferation of mesangial matrix, slight-grade proliferation of mesangial cells, segmental sclerosis of 22% of glomeruli, and overexpression of PDGF-B and bFGF in glomeruli were showed by pathologic examination, computerized image analysis and immunohistochemistry staining.Conclusions The models established in the transition stage from mesangioproliferation to glomerulosclerosis are helpful to study mechanisms and take measures for prevention and cure of chronic glomerulonephritis.
