Immunogenicity of Glutaraldehyde Tanned Bovine Pericardium with Polydiol-post Treatment
- VernacularTitle:经混合醇改性后的戊二醛鞣制的牛心包免疫原性测定
- Author:
Wei ZHANG
;
Guangfu GONG
;
Jianguo HU
- Publication Type:Journal Article
- Keywords:
Glutaraldhyde;
Polydiol;
Bovine Pericardium;
Immunogenicity S.P;
Animal experiment,rat
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this comparative study, we used a new immunocellular chemistry technique,Biotin- Streptavidin-Peroxidase(S.P) method to test the immunologic response to glutaraldehyde-pretreatment or polydiol-post treatment bovine pericardium in vitro.Method S. P was used to measure the immunologic responses to three group of bovine pericardium.Results The results of S. P in fresch bovine pericardium group showed strongly iummunogenicity in vitro. Glutaraldehyde-pretreated or polydiol-post treated bovine pericardium showed less immunogenicity in vitro.Conclusions ⑴The immunogenicity of G-tBP with polydiol post-treated was reduced significantly.⑵ S.P is a new technique and sensitive method for testing the immunogenicity of bioprosthesis in vitro.
