Observation on Relationship Between Different Serum Markers Model for Patients with Hepatitis B Virus Infection and HBV-DNA Quantitative Analysis
- VernacularTitle:乙型肝炎病毒感染者不同血清学标志物模式与HBV-DNA定量关系的研究
- Author:
Keyu LIAO
;
Zhigao PEN
;
Chanben XIONG
- Publication Type:Journal Article
- Keywords:
Hepatitis virus;
B type;
Quantum-PCR;
DNA
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between different serum markers model for patients with HBV infection and content of HBV-DNA,to provide evidence of diagnosis,treatment and provention of hepatitis B.Methods Determinations of HBV serum markers and HBV-DNA content by ELISA and real time fluorometric quantitative-PCR technique respectively in 814 sera samples.Results HBV-DNA positive rate was 87 28%(302 of 346 coses) in HBsAg(+),HBeAg(+),HBcAb(+) model.HBV copy quantity ≥10 8/ml accounted for 63 87%.HBV-DNA positive rate was 48 778%(40 of 82 cases) in HBcAb positive model HBV-DNA copy quantity in the partial samples was higher,it can occure in gene mutation of HBV precore regions,resulting in the experssion of HBeAg was failed,but virus replication was not influenced.The HBV-DNA positive rate and copy quantity in HBeAg(+) model was obviously high than those of other different serum markers model.Conclusion Serum marker model only applied to judg the degree of HBV replication and its infectivity were considerable diffecult,HBV-DNA quantitative determination could real reflect HBV replication condition,provede objective evidences of diagnosis,prevention and treatment of HBC hepatitis.
