Establishment of ELISA Method of Detecting Human Cytomegalovirus Using Synthetic Peptide and its Application
- VernacularTitle:合成肽检测巨细胞病毒酶联免疫方法的建立及应用
- Author:
Yinping LU
;
Jihua DONG
;
Wei CAO
- Publication Type:Journal Article
- Keywords:
Human,cytomegalovirus;
ELISA;
Synthetic,peptide
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the ELISA method by using synthetic peptide for detection of IgM antibodies to human cytomegalovirus(HCMV).Methods According to HCMV PP150 sequence of amino acid and nucleotide,the peptide sequence was decided by computer with some program of software of protein.The ELISA method using synthetic peptide as antigen was developed and was applied to detected anti-HCMV IgM in sera of normal pregnant woman and habitual abortion woman.The HCMV DNA were detected by polymerase chain reaction(PCR).Results The positive rates of anti-HCMV IgM in different populations were as follows:9 17%(11/120) in normal pregnant woman,37 5%(24/64) in habitual abortion woman.The ELISA method have good speciality.The positive rate of HCMV DNA was significantly related to that of anti-HCMV IgM(P
