TNF?, ConA and mechanical injury stimulate matrix metalloproteinase-9 expression in human bronchial epithelial cell line h292
- VernacularTitle:肿瘤坏死因子-?、刀豆素A及机械损伤刺激人支气管上皮细胞系H292表达基质金属蛋白酶-9
- Author:
Zhaosheng HAN
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
Bronchi;
Epithelial cells;
Tumor necrosis factor;
Concanavalin A;
Mechanical injuries;
Gelatinase B
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression of matrix metalloproteinase-9 (MMP-9) in human bronchial epithelial cell treated with TNF-?, ConA and mechanical injury. METHODS: RT-PCR and gelatin zymography were performed to observe the dynamic expression of matrix metalloproteinase-9(MMP-9) in human bronchial epithelial cell line H292 at different timepoint(2 h, 6 h,12 h, 24 h) after stimulation with TNF-?, ConA and mechanical injury. RESULTS: Human bronchial epithelial cell H292 expressed and secreted matrix metalloproteinase-9 in the presence of TNF-?, ConA and mechanical injury. On stimulation with mechanical injury alone, MMP-9 was initially expressed at 2 h, peaked at 12 h, and decreased at 24 h. Furthermore, on stimulation with combination of ConA and mechanical injury, the MMP-9 mRNA expression was the highest among in all the groups. Zymography show that the MMP-9 activity appeared just at 24 h timepoint, and was highest at the group of combination of ConA and mechanical injury. CONCLUTION: Human bronchial epithelial cell expressed and secreted MMP-9 when treated with TNF-?, ConA and mechanical injury.
