Human mesenchymal stem cells differentiate into osteoblasts
- VernacularTitle:体外定向诱导人骨髓间质干细胞分化为成骨细胞的研究
- Author:
Lirong ZHANG
;
Wenjie XIA
;
Peng XIANG
;
Zhenguang CHEN
;
Xiuming ZHANG
;
Yan LI
;
Shunon LI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Osteoblasts
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the differentiation from human mesenchymal stem cells (hMSC) into osteoblasts. METHODS: MSC were separated from human marrow with Ficoll-Paque reagent and expanded in cuture medium. To detect the surface antigens, The labeled cells were analysed on a FACScan flow cytometer. hMSC were induced to differentiate from mesenchymal stem cells into osteoblasts with dexamethasone, vitamin C, ?-GP. Cell morphology?AP activity?calcium deposition and osteopontin were detected. P10 MSC were compared to P3 MSC in the tendency of osteoblastic differentiation. RESULTS: The cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. hMSC showed a strong self-renewal capacity. After primary culture, approximately (5-6)?10 5 cells were obtained. These expanded attached MSC were uniformaly positive for CD29,CD44,CD59,CD105,CD166 and didn't express CD11a, CD14, CD33, CD34, CD45, CD38, CD80, CD86, CD117. After osteoblasts induction, the cells changed from spindle-shape to cuboidal and polygonal in cell morphology. The AP activity increased gradually and many scattered calcium nodes were observed. The expression of osteopontin was positive. CONCLUSION: hMSC can be induced to differentiate into osteoblasts.
