Influence of different flow pattern on VCAM-1, NF-?B expression in vascular endothelial cells
- VernacularTitle:流体的不同流动方式对血管内皮细胞血管细胞粘附分子-1、核因子?B表达的影响
- Author:
Guanqing QIAN
;
Huiqi LIU
;
Xiaohui LIU
- Publication Type:Journal Article
- Keywords:
Endothelium, vascular;
Hemodynamics;
Transcription factors
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:[WTBZtive of this study was to examine the influence of different flow pattern, particularly low shear stress flow regimens, on VCAM-1 and NF-?B in vascular endothelial cells. [WTHZ] METHODS: HUVEC covered sheets were placed within rectangular parallel plate flow chambers. Cells were subjected to 4, 18 h of either laminar or oscillatory shear stress. The expression of VCAM-1 and NF-?B activity were analyzed by immunohistochemistry. VCAM-1 mRNA was also detected using in situ hybridization. RESULTS: VCAM-1 mRNA and VCAM-1 expression were not upregulated by laminar shear stress. However, oscillatory shear stress significantly upregulated VCAM-1 mRNA and VCAM-1 expression. Translocation of NF-?B P65 was remarkably increased. CONCLUSION: In same low shear stress, the different expression of VCAM-1 mRNA and VCAM-1 were induced by HUVEC exposed to laminar or oscillatory flow patterns.
