Effect of protein kinase C-? antisense oligonucleotide on cell cycle of CNE-2Z cells
- VernacularTitle:反义PKC?对CNE-2Z细胞周期的影响
- Author:
Bo BAO
;
Peichun HUANG
;
Chuanren DONG
;
Jin CHEN
;
Nanyue CHEN
;
Zhiwe HE
- Publication Type:Journal Article
- Keywords:
Protein kinase C;
Nasopharyngeal neoplasms;
Cell cycle;
Oligonucleotides, antisense
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of protein kinase C-?(PKC?)antisense oligonucleotide on cell growth, cell cycle and the expression of cyclin E in human poor-differentiated nasopharyngeal carcinoma(NPC) cell line CNE-2Z. METHODS: Antisense PKC? was transfected by cationic liposome(LP) in CNE-2Z cells to analyze the cell growth and cell cycle by MTT colorimetric assay and flow cytometry, respectively. Moreover, the expression of cyclin E was determined by immunocellularchemistry and scanning the result of dot-blotting. RESULTS: ①With the concentration of antisense PKC? increasing, the relative cell growth index was decreased gradually( P
