Model of primary cultured neuron injury induced by D-galactose
- VernacularTitle:D-半乳糖致原代培养神经元损伤模型的研究
- Author:
Dayong CAI
;
Yan ZHAO
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Galactose;
Neurons;
Rats;
Alzheimer's disease
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a model of primary cultured neuron injury induced by D-galactose for the research in Alzheimer's disease. METHODS: Primary rat neurons cultured for 6 days were exposed to 50 mmol D-galactose for 72 h. The neural growth and neurite density were observed with HE stain and microscope, the neural metabolism rate and apoptosis rate were examined with MTT, immuno-enzyme assay and flow cytometry, respectively, and the aldose reductase mRNA expression was also detected with RT-PCR. RESULTS: The neural growth and development in neurons treated with D-galactose was retarded, the neural metabolism rate decreased from 0.762?0 030( n= 33) to 0 543?0 064( n= 11)( P
