In vitro effect of cytosine deaminase gene therapy and chemical reagents on colon cancer cell line
- VernacularTitle:自杀基因联合化疗药物治疗结肠癌的体外研究
- Author:
Lizong SHEN
;
Qiang DING
;
Wenxi WU
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Genes;
Drug therapy
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the effect of combined cytosine deaminase(CD) gene therapy and chemical reagents on colon cancer cell line.MethodsLovo cells transducted with CD gene in a recombinant adenovirus AdCMVCD vector were treated with 5 fluorocytosine(5 FC) and ciplatin(CDDP) or mitomycine C(MMC). Cytotoxic effect was assayed with MTT method. ResultsLow dose of CDDP or MMC reduces the IC 50 of 5 FC significantly on Lovo cells transducted with CD gene and enhances the bystander effect of AdCMVCD/5 FC system.ConclusionCombined therapy with CD/5 FC system and chemical reagents may have a potential application in the treatment of colon cancer.
