Antitumor efficacy of lymphocytes from hepatic porta lymph node stimulated by staphylococcal enterotoxin A
- VernacularTitle:葡萄球菌肠毒素A诱导原发性肝细胞癌患者肝门淋巴结细胞抗癌活性的研究
- Author:
Hua XUE
;
Zhiyu LI
;
Sheng HE
- Publication Type:Journal Article
- Keywords:
Enterotoxins;
Lymphocytes;
Lymph nodes;
Carcinoma, hepatocellular
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the antitumor effects of lymphocytes from hepatic porta lymph node co cultured with staphylococcal enterotoxin A(SEA)?MethodsLymphocytes isolated from lymph node of 5 HCC patients were co cultured with SEA. The proliferation and cytotoxicity of the lymphocytes were observed. Flow cytometer was used to detect the expression of CD3?CD4 and CD8 on the surface of the lymphocytes. TNF ? and IFN ? released into the culture medium were measured by the method of ELISA. The results were compared with that yielded from TILs of the individual patients. ResultsCultured in the presence of SEA, there was a sharp rise of T cells, especially CD8 + cells. Cytotoxic activity was increased. TNF ? and IFN ? were predominantly produced in the first week.ConclusionsLymphocytes from lymph node activated by SEA were armed with an increased antitumor efficacy.
