Isolation and Cultivation of Cochlear Microvascular Endothelial Cells from Guinea Pigs by Tissue Nubbles Cultivation and Collagenase Digestion.
- VernacularTitle:组织块培养法及胶原酶消化法分离培养豚鼠耳蜗微血管内皮细胞
- Author:
Yunjun WEI
;
Xueyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Guinea pig Striae vascularis Endothelial cell Cell culture Immunohistochemical stains
- From:
Journal of Audiology and Speech Pathology
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up reliable cell culture methods of cochlear microvascular endothelial cells in guinea pigs.Methods The cochlear striae vascularies were acquired from guinea pigs with micrergy, the cells were cultured and pured by tissue nubbles cultivation and collagenase digestion.Results Two days after tissue nubbles cultivation of striae vascularis, cells grew at the edge of some tissue nubbles,and proliferated to massive colonies composed of more cells;Some islands made up of a few cells on the bottom of Petri dish were found one to three days after striae vascularies tissue was digested by collagenase;These cells were mainly polygon after purification, monolayer confluent culture cells showed typical "flagstone" appearance as cul tured endothelial cells. According to immunohistochemical detection of factor VIII, which is the mark antigen of endothelial cell, we had observed that the positive reaction cells with cytoplasmic brown pigment were over 95%.Conclusion Cochlear microvascular endothelial cells from guinea pigs can be acquired by tissue nubbles cultivation and collagenase digestion .
